Category: Rheumatology
Test Code: 052
Disease: • Antibodies to Extractable Nuclear (ENA) & Cytoplasmic Antigens • Dermato/Polymyositis
Methodology: ELISA
Reference Range: Negative: <20; Borderline: 20-25; Positive: >25
Units: EU/ml
CPT Code: 86235
Schedule / Turnaround Time: Assay performed once weekly. Report availability is within one week from the time of specimen receipt.
Specimen Requirements:

Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.

Sample Stability:

Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year.


These antibodies are associated with a high frequency of myositis-scleroderma overlap.