Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.

Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year.

pANCA occurs in vasculitis, glomerulonephritis, Churg-Strauss syndrome, polyarteritis nodosa, systemic lupus erythematosus, and rheumatoid arthritis. A major antigen of pANCA is myeloperoxidase (MPO), which constitutes a potent microbicidal system within the neutrophil granulocytes. Additional target antigens such as human leukocyte elastase, and lactoferrin have also been associated with the pANCA fluorescence pattern. Antibodies to MPO can also be induced by drugs such as hydralazine, clozapine, and L-tryptophan. Occupational exposure to environmental factors such as silica dust may provoke an anti-MPO positive progressive glomerulonephritis. Measurement of MPO specific ANCA is an important aid in the evaluation of clinical subtypes within systemic vasculitides. Clinical Relevance: pANCA occurs in vasculitis, glomerulonephritis, Churg-Strauss syndrome, polyarteritis nodosa, systemic lupus erythematosus, and rheumatoid arthritis. A major antigen of pANCA is myeloperoxidase (MPO), which constitutes a potent microbicidal system within the neutrophil granulocytes. Additional target antigens such as human leukocyte elastase, and lactoferrin have also been associated with the pANCA fluorescence pattern. Antibodies to MPO can also be induced by drugs such as hydralazine, clozapine, and L-tryptophan. Occupational exposure to environmental factors such as silica dust may provoke an anti-MPO positive progressive glomerulonephritis. Measurement of MPO specific ANCA is an important aid in the evaluation of clinical subtypes within systemic vasculitides. Clinical Relevance: pANCA occurs in vasculitis, glomerulonephritis, Churg-Strauss syndrome, polyarteritis nodosa, systemic lupus erythematosus, and rheumatoid arthritis. A major antigen of pANCA is myeloperoxidase (MPO), which constitutes a potent microbicidal system within the neutrophil granulocytes. Additional target antigens such as human leukocyte elastase, and lactoferrin have also been associated with the pANCA fluorescence pattern. Antibodies to MPO can also be induced by drugs such as hydralazine, clozapine, and L-tryptophan. Occupational exposure to environmental factors such as silica dust may provoke an anti-MPO positive progressive glomerulonephritis. Measurement of MPO specific ANCA is an important aid in the evaluation of clinical subtypes within systemic vasculitides.