dsDNA (nDNA) antibody titer

Category: Rheumatology
Test Code: 004
Disease: Systemic Lupus Erythematosus (SLE)
Methodology: Indirect Immunofluorescence
Substrate: Crithidia luciliae Double Stranded DNA Antibody (nDNA) IgG
Reference Range: Negative: < 1:10
Units: Titer
Note: Positive samples at a 1:10 screening dilution are titered to an endpoint at an additional charge.
CPT Code: 86255
Schedule / Turnaround Time: Assay performed daily Monday-Friday. Report availability is within 48 hours from the time of specimen receipt.
Specimen Requirements: Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.
Sample Stability: Sample is stable at ambient temperature during shipment. If sample is stored prior to shipment, it is stable refrigerated (2-8˚C) up to five days and frozen (-20˚C or lower) up to one year.
Relevance: nDNA are highly specific for systemic lupus erythematosus. The frequency and titer of nDNA antibodies fluctuate with disease activity and tend to disappear with immunosuppressive treatment and during remission.