Gastroenterology & Hepatology
|Disease:||Inflammatory Bowel Disease (IBD)|
|Methodology:||Indirect Immunofluorescence, ELISA and Nephelometry|
|CPT Code:||86038, 86431(x3), 82784 (x1) 82778 (x1)|
|Schedule / Turnaround Time:||See individual tests for scheduling and turnaround time.|
Specimen need not be refrigerated or frozen. Collect 2-3 ml of blood in a red top or serum separator tube. If possible, separate serum from clot and place into white tube provided with Immco Diagnostics’ collection kits. If separation facilities are not available, the blood can be sent in the tube used for collection.
Sample is stable at ambient temperature during shipment. If ample is stored prior to shipment, it is stable at refrigerated (2-8 degree centigrade) up to five days and frozen (-20 degree centigrade) up to a year.
The elevated serum IgG4 level is nearly diagnostic of AIP. IgG4 shows specificity of 98% and sensitivity of 80-92% when cutoff values of 135mg/dl are used. IgG levels are also elevated and shows specificity of 73% and sensitivity of 75%. Antinuclear antibodies (ANA) and rheumatoid factor (RF) levels are also increased and show a lower sensitivity rate. However a combination of IgG4, IgG, ANA and RF can increase the sensitivity rate up to 97%.