Lipase

The Trinity Biotech Lipase: PS kit is intended for use in the quantitative, kinetic determination of pancreatic lipase (triacylglycerol acylhydrolase) activity in serum.

The measurement of serum lipase activity is widely used for the diagnosis of acute pancreatitis. A 10-fold increase of lipase activity above the upper reference limit is suggestive of pancreatitis, pancreatic injury, or inflammation of organs contiguous to the pancreas.


Principle

The rate of increase in absorbance at 550 nm due to the formation of quinone diimine dye is directly proportional to the lipase activity in the sample.


Diagnostic Implications

A 10-fold increase of lipase activity above the upper reference limit is suggestive of pancreatitis, pancreatic injury, or inflammation of organs contiguous to the pancreas. it is recommended that other tests, such as trypsinogen and amylase isosenzymes be performed to supplement the diagnosis.