Clinical Chemistry

Oxalate

Trinity Biotech’s Oxalate kit is intended for the quantitative, enzymatic determination of oxalate in urine. Trinity Biotech can provide complete kits and individual components for assaying oxalate concentration. A decreased excretion of oxalate in the urine is associated with hyperglycinemia or hyperglycinuria while an increased excretion of oxalate in the urine can be attributed to an increase in ingestion of oxalate precursors or oxalate rich foods.

Absorption of oxalate occurs in a number of gastrointestinal disorders that produce severe fat malabsorption. This is indicative of patients with inflammatory bowel disease, ideal resection, biliary diversion, pancreatic insufficiency, small intestinal stasis with bacterial overgrowth, and following jejunoileal bypass or resection for the treatment of obesity.

Principle

Indamine dye has an absorbance maximum of 590nm. The intensity of the colour produced is directly proportional to the concentration of Oxalate in the sample.

Diagnostic Implications

A decreased excretion of oxalate in the urine is associated with hyperglycinemia or hyperglycinuria

An increased excretion of oxalate in the urine can be attributed to:

  • Increases in ingestion of oxalate precursors or oxalate rich foods
  • Formation of oxalate due to metabolic defects such as in primary hyperoxaluria
  • Absorption of oxalate in a number of gastrointestinal disorders that produce severe fat malabsorption. This is indicative of patients with inflammatory bowel disease, ideal resection, biliary diversion, pancreatic insufficiency, small intestinal stasis with bacterial overgrowth and following jejunoileal bypass or resection for the treatment of obesity.